Fast Resonance Assignment and Fold Determination of Human Superoxide Dismutase by High-Resolution Proton-Detected Solid-State MAS NMR Spectroscopy
Knight, Michael J.; Webber, Amy L.; Pell, Andrew J.; Guerry, Paul; Barbet-Massin, Emeline; Bertini, Ivano; Felli, Isabella C.; Gonnelli, Leonardo; Pierattelli, Roberta; Emsley, Lyndon; Lesage, Anne; Herrmann, Torsten; Pintacuda, Guido
2011

Abstract

Re-protonation is key: A combination of a high magnetic field (1 GHz) and ultra-fast magic-angle spinning (60 kHz) allows easy detection of NMR spectra revealing details of secondary and tertiary structures of medium-sized proteins. The technique was applied to the 153-residue microcrystalline Zn II-loaded superoxide dismutase (ZnII-SOD) fully [ 2H,13C,15N]-labeled and 100% re-protonated at the exchangeable sites. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Details

Title Fast Resonance Assignment and Fold Determination of Human Superoxide Dismutase by High-Resolution Proton-Detected Solid-State MAS NMR Spectroscopy
Author(s) Knight, Michael J. ; Webber, Amy L. ; Pell, Andrew J. ; Guerry, Paul ; Barbet-Massin, Emeline ; Bertini, Ivano ; Felli, Isabella C. ; Gonnelli, Leonardo ; Pierattelli, Roberta ; Emsley, Lyndon ; Lesage, Anne ; Herrmann, Torsten ; Pintacuda, Guido
Published in Angewandte Chemie-International Edition
Volume 50
Issue 49
Pages 11697-11701
Date 2011
Publisher WILEY-BLACKWELL
ISSN 1433-7851
Keywords
DOI https://doi.org/10.1002/anie.201106340
Other identifier(s) View record in Web of Science
Laboratories LRM
Record Appears in Scientific production and competences > SB - School of Basic Sciences > ISIC - Institute of Chemical Sciences and Engineering > LRM - Laboratory of Magnetic Resonance
Peer-reviewed publications
Work outside EPFL
Journal Articles
Published
Record creation date 2015-01-08

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