research article
Fast Resonance Assignment and Fold Determination of Human Superoxide Dismutase by High-Resolution Proton-Detected Solid-State MAS NMR Spectroscopy
Re-protonation is key: A combination of a high magnetic field (1 GHz) and ultra-fast magic-angle spinning (60 kHz) allows easy detection of NMR spectra revealing details of secondary and tertiary structures of medium-sized proteins. The technique was applied to the 153-residue microcrystalline Zn II-loaded superoxide dismutase (ZnII-SOD) fully [ 2H,13C,15N]-labeled and 100% re-protonated at the exchangeable sites. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Type
research article
Web of Science ID
WOS:000298084900024
Author(s)
Knight, Michael J.
Webber, Amy L.
Pell, Andrew J.
Guerry, Paul
Barbet-Massin, Emeline
Bertini, Ivano
Felli, Isabella C.
Gonnelli, Leonardo
Pierattelli, Roberta
Date Issued
2011
Publisher
Published in
Volume
50
Issue
49
Start page
11697
End page
11701
Editorial or Peer reviewed
REVIEWED
Written at
OTHER
EPFL units
Available on Infoscience
January 8, 2015
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