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research article
Fast Resonance Assignment and Fold Determination of Human Superoxide Dismutase by High-Resolution Proton-Detected Solid-State MAS NMR Spectroscopy
Re-protonation is key: A combination of a high magnetic field (1 GHz) and ultra-fast magic-angle spinning (60 kHz) allows easy detection of NMR spectra revealing details of secondary and tertiary structures of medium-sized proteins. The technique was applied to the 153-residue microcrystalline Zn II-loaded superoxide dismutase (ZnII-SOD) fully [ 2H,13C,15N]-labeled and 100% re-protonated at the exchangeable sites. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Type
research article
Web of Science ID
WOS:000298084900024
Authors
Knight, Michael J.
•
Webber, Amy L.
•
Pell, Andrew J.
•
Guerry, Paul
•
Barbet-Massin, Emeline
•
Bertini, Ivano
•
Felli, Isabella C.
•
Gonnelli, Leonardo
•
Pierattelli, Roberta
•
Publication date
2011
Publisher
Published in
Volume
50
Issue
49
Start page
11697
End page
11701
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
January 8, 2015
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