Fast Resonance Assignment and Fold Determination of Human Superoxide Dismutase by High-Resolution Proton-Detected Solid-State MAS NMR Spectroscopy

Re-protonation is key: A combination of a high magnetic field (1 GHz) and ultra-fast magic-angle spinning (60 kHz) allows easy detection of NMR spectra revealing details of secondary and tertiary structures of medium-sized proteins. The technique was applied to the 153-residue microcrystalline Zn II-loaded superoxide dismutase (ZnII-SOD) fully [ 2H,13C,15N]-labeled and 100% re-protonated at the exchangeable sites. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Published in:
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 50, 49, 11697-11701
Year:
2011
Publisher:
WILEY-BLACKWELL
ISSN:
1433-7851
Keywords:
Laboratories:




 Record created 2015-01-08, last modified 2018-03-17


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