Fast Resonance Assignment and Fold Determination of Human Superoxide Dismutase by High-Resolution Proton-Detected Solid-State MAS NMR Spectroscopy
Re-protonation is key: A combination of a high magnetic field (1 GHz) and ultra-fast magic-angle spinning (60 kHz) allows easy detection of NMR spectra revealing details of secondary and tertiary structures of medium-sized proteins. The technique was applied to the 153-residue microcrystalline Zn II-loaded superoxide dismutase (ZnII-SOD) fully [ 2H,13C,15N]-labeled and 100% re-protonated at the exchangeable sites. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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Knight, M. J., Webber, A. L., Pell, A. J., Guerry, P., Barbet-Massin, E., Bertini, I., Felli, I. C., Gonnelli, L., Pierattelli, R., Emsley, L., Lesage, A., Herrmann, T., & Pintacuda, G. (2011). Fast Resonance Assignment and Fold Determination of Human Superoxide Dismutase by High-Resolution Proton-Detected Solid-State MAS NMR Spectroscopy. Angewandte Chemie International Edition, 50(49), 11697–11701. https://doi.org/10.1002/anie.201106340
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WOS:000298084900024
2011
50
49
11697
11701
REVIEWED
OTHER