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research article
Backbone Assignment of Fully Protonated Solid Proteins by 1H Detection and Ultrafast Magic-Angle-Spinning NMR Spectroscopy
Narrow 1H NMR linewidths can be obtained for fully protonated protein samples in the solid state by using ultrafast magic-angle spinning (60 kHz). Medium-size microcrystalline and noncrystalline proteins can be analyzed without any need for deuteration of the protein sample. This approach provides assignments of the backbone 1H, 15N, 13C α, and 13CO resonances and yields information about 1H-1H proximities. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Type
research article
Web of Science ID
WOS:000310076100012
Authors
Marchetti, Alessandro
•
Jehle, Stefan
•
Felletti, Michele
•
Knight, Michael J.
•
Wang, Yao
•
Xu, Zhi-Qiang
•
Park, Ah Young
•
Otting, Gottfried
•
Lesage, Anne
•
Publication date
2012
Publisher
Published in
Volume
51
Issue
43
Start page
10756
End page
10759
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
January 8, 2015
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