Backbone Assignment of Fully Protonated Solid Proteins by 1H Detection and Ultrafast Magic-Angle-Spinning NMR Spectroscopy

Narrow 1H NMR linewidths can be obtained for fully protonated protein samples in the solid state by using ultrafast magic-angle spinning (60 kHz). Medium-size microcrystalline and noncrystalline proteins can be analyzed without any need for deuteration of the protein sample. This approach provides assignments of the backbone 1H, 15N, 13C α, and 13CO resonances and yields information about 1H-1H proximities. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Published in:
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 51, 43, 10756-10759
Year:
2012
Publisher:
WILEY-V C H VERLAG GMBH
ISSN:
1433-7851
Keywords:
Laboratories:




 Record created 2015-01-08, last modified 2018-03-17


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