Backbone Assignment of Fully Protonated Solid Proteins by 1H Detection and Ultrafast Magic-Angle-Spinning NMR Spectroscopy
2012
Abstract
Narrow 1H NMR linewidths can be obtained for fully protonated protein samples in the solid state by using ultrafast magic-angle spinning (60 kHz). Medium-size microcrystalline and noncrystalline proteins can be analyzed without any need for deuteration of the protein sample. This approach provides assignments of the backbone 1H, 15N, 13C α, and 13CO resonances and yields information about 1H-1H proximities. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Details
Title
Backbone Assignment of Fully Protonated Solid Proteins by 1H Detection and Ultrafast Magic-Angle-Spinning NMR Spectroscopy
Author(s)
Marchetti, Alessandro ; Jehle, Stefan ; Felletti, Michele ; Knight, Michael J. ; Wang, Yao ; Xu, Zhi-Qiang ; Park, Ah Young ; Otting, Gottfried ; Lesage, Anne ; Emsley, Lyndon ; Dixon, Nicholas E. ; Pintacuda, Guido
Published in
Angewandte Chemie-International Edition
Volume
51
Issue
43
Pages
10756-10759
Date
2012
Publisher
WILEY-V C H VERLAG GMBH
ISSN
1433-7851
Keywords
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Laboratories
LRM
Record Appears in
Scientific production and competences > SB - School of Basic Sciences > ISIC - Institute of Chemical Sciences and Engineering > LRM - Laboratory of Magnetic Resonance
Peer-reviewed publications
Work outside EPFL
Journal Articles
Published
Peer-reviewed publications
Work outside EPFL
Journal Articles
Published
Record creation date
2015-01-08