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  4. Out-and-back C-13-C-13 scalar transfers in protein resonance assignment by proton-detected solid-state NMR under ultra-fast MAS
 
research article

Out-and-back C-13-C-13 scalar transfers in protein resonance assignment by proton-detected solid-state NMR under ultra-fast MAS

Barbet-Massin, Emeline
•
Pell, Andrew J.
•
Jaudzems, Kristaps
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2013
JOURNAL OF BIOMOLECULAR NMR

We present here H-1-detected triple-resonance H/N/C experiments that incorporate CO-CA and CA-CB out-and-back scalar-transfer blocks optimized for robust resonance assignment in biosolids under ultra-fast magic-angle spinning (MAS). The first experiment, (H)(CO)CA(CO)NH, yields H-1-detected inter-residue correlations, in which we record the chemical shifts of the CA spins in the first indirect dimension while during the scalar-transfer delays the coherences are present only on the longer-lived CO spins. The second experiment, (H)(CA)CB(CA)NH, correlates the side-chain CB chemical shifts with the NH of the same residue. These high sensitivity experiments are demonstrated on both fully-protonated and 100 %-H-N back-protonated perdeuterated microcrystalline samples of Acinetobacter phage 205 (AP205) capsids at 60 kHz MAS.

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Type
research article
DOI
10.1007/s10858-013-9757-3
Web of Science ID

WOS:000323661800009

Author(s)
Barbet-Massin, Emeline
Pell, Andrew J.
Jaudzems, Kristaps
Franks, W. Trent
Retel, Joren S.
Kotelovica, Svetlana
Akopjana, Inara
Tars, Kaspars
Emsley, Lyndon  
Oschkinat, Hartmut
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Date Issued

2013

Publisher

SPRINGER

Published in
JOURNAL OF BIOMOLECULAR NMR
Volume

56

Issue

4

Start page

379

End page

386

Subjects

Fully-protonated and deuterated biosolids

•

Solid-state NMR

•

Ultra-fast magic-angle spinning

•

Through-bond transfers

•

Sensitivity

Editorial or Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
LRM  
Available on Infoscience
January 8, 2015
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/109970
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