Time-resolved imaging system for fluorescence-guided surgery with lifetime imaging capability

• Presented at: Conference on Biophotonics - Photonic Solutions for Better Health Care IV, Brussels, BELGIUM, APR 14-17, 2014
• Published in: Biophotonics: Photonic Solutions For Better Health Care Iv (ISSN: 0277-786X) (ISBN: 978-1-62841-077-8), vol. 9129
• Series: Proceedings of SPIE
• Bellingham: Spie-Int Soc Optical Engineering, 2014

We present a single-photon camera for fluorescence imaging, with a time resolution better than 100ps, capable of providing both intensity and lifetime images. the camera was fabricated in standard CMOS technology. With this FluoCam we show the possibility to study sub-nanosecond fluorescence mechanisms. The FluoCam was used to characterize a near-infrared probe, indocyanine green, conjugated with multimeric cyclic pentapeptide ( cRGD). The fluorescent probe-conjugated was used to target and mark tumors with better specificity, in particular aiming at targeting the integrins alpha(v)beta(3) and alpha(v)beta(5). As a first step towards clinical studies, preliminary results obtained in-vivo are presented. The first envisioned clinical application would be image-guided surgical oncology to help the surgeon to remove tumor tissue by a better discrimination from normal tissues and also to improve the detection of metastatic lymph nodes. A further application could be the in-vivo determination of the alpha(v)beta(3) and alpha(v)beta(5) targets to select patients for therapy with RGD-chemotherapy conjugates.

Keywords: Single Photon Avalanche Diodes (SPADs) ; Fluorescence Lifetime Imaging ; Indocyanine green (ICG) ; Image-guided surgery

Reference

• EPFL-CONF-204199
• doi:10.1117/12.2052411
• View record in Web of Science

Record created on 2014-12-30, modified on 2017-05-10