Yeast Three-Hybrid Screening for Identifying Anti-Tuberculosis Drug Targets

• Published in: Chembiochem (ISSN: 1439-4227), vol. 14, num. 17, p. 2239-2242
• Weinheim: Wiley-V C H Verlag Gmbh, 2013

Mycobacterium goes yeast: Target deconvolution of anti-tuberculosis drugs can be a very challenging task. Here we report a yeast 3-hybrid system that allows promising small molecules to be screened for protein targets of a pathogen in nontoxic yeast cells. The system employs libraries of randomly fragmented bacterial DNA and offers a technically simple alternative approach for target identification. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords: genomic library ; snap-tag ; target deconvolution ; tuberculosis ; yeast three-hybrid system

Reference

• EPFL-ARTICLE-199162
• doi:10.1002/cbic.201300472
• View record in Web of Science

Record created on 2014-06-02, modified on 2017-07-28