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  4. Linking toxicity and adaptive responses across the transcriptome, proteome, and phenotype of Chlamydomonas reinhardtii exposed to silver
 
research article

Linking toxicity and adaptive responses across the transcriptome, proteome, and phenotype of Chlamydomonas reinhardtii exposed to silver

Pillai, Smitha
•
Behra, Renata
•
Nestler, Holger
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2014
Proceedings Of The National Academy Of Sciences Of The United States Of America (PNAS)

Understanding mechanistic and cellular events underlying a toxicological outcome allows the prediction of impact of environmental stressors to organisms living in different habitats. A systems-based approach aids in characterizing molecular events, and thereby the cellular pathways that have been perturbed. However, mapping only adverse outcomes of a toxicant falls short of describing the stress or adaptive response that is mounted to maintain homeostasis on perturbations and may confer resistance to the toxic insult. Silver is a potential threat to aquatic organisms because of the increasing use of silver-based nanomaterials, which release free silver ions. The effects of silver were investigated at the transcriptome, proteome, and cellular levels of Chlamydomonas reinhardtii. The cells instigate a fast transcriptome and proteome response, including perturbations in copper transport system and detoxification mechanisms. Silver causes an initial toxic insult, which leads to a plummeting of ATP and photosynthesis and damage because of oxidative stress. In response, the cells mount a defense response to combat oxidative stress and to eliminate silver via efflux transporters. From the analysis of the perturbations of the cell's functions, we derived a detailed mechanistic understanding of temporal dynamics of toxicity and adaptive response pathways for C. reinhardtii exposed to silver.

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Type
research article
DOI
10.1073/pnas.1319388111
Web of Science ID

WOS:000332560300068

Author(s)
Pillai, Smitha
Behra, Renata
Nestler, Holger
Suter, Marc J-F
Sigg, Laura
Schirmer, Kristin  
Date Issued

2014

Publisher

National Academy of Sciences

Published in
Proceedings Of The National Academy Of Sciences Of The United States Of America (PNAS)
Volume

111

Issue

9

Start page

3490

End page

3495

Subjects

toxicity response

•

adaptive pathway

•

algae

•

systems biology

•

adverse outcome pathway

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
TOX  
Available on Infoscience
May 2, 2014
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/103140
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