Reduced Dyes Enhance Single-Molecule Localization Density for Live Superresolution Imaging

Cell-permeable rhodamine dyes are reductively quenched by NaBH4 into a non-fluorescent leuco-rhodamine form. Quenching is reversible, and their fluorescence is recovered when the dyes are oxidized. In living cells, oxidation occurs spontaneously, and can result in up to ten-fold higher densities of single molecule localizations, and more photons per localization as compared with unmodified dyes. These two parameters directly impact the achievable resolution, and we see a significant improvement in the quality of live-cell point-localization super-resolution images taken with reduced dyes. These improvements carry over to increase the density of trajectories for single-molecule tracking experiments.


Published in:
Chemphyschem, 15, 4, 750-755
Year:
2014
Publisher:
Weinheim, Wiley-V C H Verlag Gmbh
ISSN:
1439-4235
Keywords:
Laboratories:




 Record created 2014-05-02, last modified 2018-03-17


Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)