Apart from their contribution in the water balance of Lake Ohrid, Republic of Macedonia, subaquatic springs are also expected to affect the water quality. A simple experiment was developed and applied to subaquatic spring in Kališta region (south-east of Kališta village, in the north-western part of the Lake) and at the spring at Veli Dab (eastern side of the Lake) based on physicochemical spring water properties. Different sampling methods were established with the aim of uncovering a more suitable way of sampling pure subaquatic spring water. The goal was to test these findings and adapt them for further analysis with higher temporal and measurement related resolution. Measurements were also aimed at gathering additional knowledge and methodology for characterizing the hydrogeology of the watersheds. Of interest was information on the general chemical composition of spring water, interactions between aquifer and groundwater and on the origins of groundwater. Integration of this knowledge adds to a better understanding on how and what kind of groundwater is delivered to Lake Ohrid. The obtained results show that the examined springs are rich in nutrients and, comparing to Lake water, exhibit temporal variations in temperature, pH, conductivity and dissolved oxygen. Investigated springs have a very constant flow, as well as water quality.