Live Intracellular Super-Resolution Imaging Using Site-Specific Stains

Point localization super-resolution imaging (SR) requires dyes that can cycle between fluorescent and dark states, in order for their molecular positions to be localized and create a reconstructed image. Dyes should also densely decorate biological features of interest to fully reveal structures being imaged. We tested site-specific dyes in several live-cell compatible imaging media and evaluated their performance in situ. We identify a number of new dyes and imaging medium-dye combinations for live staining, that densely highlight intracellular structures with excellent photophysical performance for SR.


Published in:
ACS Chemical Biology, 8, 12, 2643-2648
Year:
2013
Publisher:
Washington, American Chemical Society
ISSN:
1554-8929
Laboratories:




 Record created 2014-02-17, last modified 2018-03-17


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