Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells

Transient gene expression (TGE) from mammalian cells is an increasingly important tool for the rapid production of recombinant proteins for research applications in biochemistry, structural biology, and biomedicine. Here we review methods for the transfection of human embryo kidney (HEK-293) and Chinese hamster ovary (CHO) cells in suspension culture using the cationic polymer polyethylenimine (PEI) for gene delivery.


Published in:
Protein Expression and Purification, 92, 1, 67-76
Year:
2013
Publisher:
San Diego, Elsevier
ISSN:
1046-5928
Keywords:
Laboratories:




 Record created 2013-12-04, last modified 2018-03-17


Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)