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  4. Changes in the Bacterial Community and lin Genes Diversity during Biostimulation of Indigenous Bacterial Community of Hexachlorocyclohexane (HCH) Dumpsite Soil
 
research article

Changes in the Bacterial Community and lin Genes Diversity during Biostimulation of Indigenous Bacterial Community of Hexachlorocyclohexane (HCH) Dumpsite Soil

Gupta, Sanjay Kumar
•
Lal, Devi
•
Lata, Pushp
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2013
Microbiology

In this study hexachlorocyclohexane (HCH) contaminated soil (with HCH level 84 g/kg of soil) from HCH dumpsite (Ummari village, Lucknow, India) was used to demonstrate biostimulation approach for HCH bioremediation. Different nutrients (molasses and ammonium phosphate) were used in different pits having contaminated soil to stimulate the indigenous microbial community. There was a substantial reduction in the total HCH content of the soil in 12 months long experiment. Maximum reduction was seen in the pit that received a combination of molasses and ammonium phosphate. A change in the microbial community concomitant with degradation of HCH was observed. Sphingomonads, which are known degraders of HCH, were found to dominate the experimental pits. Moreover changes in linA and linB gene (primary genes involved in HCH degradation) diversity and number were also seen as revealed by T-RFLP and RT-PCR respectively. The study suggests the prospects of biostimulation in decontaminating soils heavily contaminated with HCH.

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Type
research article
DOI
10.1134/S0026261713020185
Web of Science ID

WOS:000320318300016

Author(s)
Gupta, Sanjay Kumar
Lal, Devi
Lata, Pushp
Sangwan, Naseer
Garg, Nidhi
Holliger, Christof  
Lal, Rup
Date Issued

2013

Publisher

Springer Verlag

Published in
Microbiology
Volume

82

Issue

2

Start page

234

End page

240

Subjects

biostimulation

•

RT-PCR

•

bacterial diversity

•

T-RFLP

•

sphingomonads

Editorial or Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
LBE  
Available on Infoscience
October 1, 2013
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/95153
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