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  4. The effect of crowding and confinement: a comparison of Yfh1 stability in different environments
 
research article

The effect of crowding and confinement: a comparison of Yfh1 stability in different environments

Sanfelice, Domenico
•
Politou, Anastasia
•
Martin, Stephen R.
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2013
Physical Biology

Crowding and confinement can affect protein stability, favouring the more compact species amongst the folded and unfolded conformations. An unbiased assessment of the relative efficacy of crowded and confined environments has been hampered so far by the paucity of homogeneous comparisons on the same protein. This paper reports spectroscopic studies on yeast frataxin (Yfh1), a protein which provides an excellent model system for stability studies since it undergoes both cold and heat denaturation at measurable temperatures. The stability of Yfh1 was evaluated in the presence of Ficoll 70 and inside the cavities of polyacrylamide gels as means of mimicking crowding and confinement. We find that both effects influence the thermal stability of Yfh1 to a comparable extent thus providing the first direct comparison of crowding and confinement on the same protein. Thanks to the measurement of the full stability curve we also present the first thermodynamic characterization of the stability of a protein in crowding conditions.

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Type
research article
DOI
10.1088/1478-3975/10/4/045002
Web of Science ID

WOS:000322701100004

Author(s)
Sanfelice, Domenico
Politou, Anastasia
Martin, Stephen R.
De Los Rios, Paolo  
Temussi, Pierandrea
Pastore, Annalisa
Date Issued

2013

Publisher

Institute of Physics

Published in
Physical Biology
Volume

10

Article Number

045002

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LBS  
Available on Infoscience
September 29, 2013
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/95014
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