Repository logo

Infoscience

  • English
  • French
Log In
Logo EPFL, École polytechnique fédérale de Lausanne

Infoscience

  • English
  • French
Log In
  1. Home
  2. Academic and Research Output
  3. Journal articles
  4. Microfluidics for in vitro biomimetic shear stress-dependent leukocyte adhesion assays
 
review article

Microfluidics for in vitro biomimetic shear stress-dependent leukocyte adhesion assays

Bianchi, Elena  
•
Molteni, Raffaella
•
Pardi, Ruggero
Show more
2013
Journal Of Biomechanics

Recruitment of leukocytes from blood to tissues is a multi-step process playing a major role in the activation of inflammatory responses. Tethering and rolling of leukocytes along the vessel wall, followed by arrest and transmigration through the endothelium result from chemoattractant-dependent signals, inducing adhesive and migratory events. Shear forces exerted by the blood flow on leukocytes induce rolling via selectin-mediated interactions with endothelial cells and increase the probability of leukocytes to engage their chemokine receptors, facilitating integrin activation and consequent arrest. Flow-derived shear forces generate mechanical stimuli concurring with biochemical signals in the modulation of leukocyte-endothelial cell interactions. In the last few years, a host of in vitro studies have clarified the biochemical adhesion cascade and the role of shear stress in leukocyte extravasation. The limitation of the static environment in Boyden devices has been overcome both by the use of parallel-plate flow chambers and by custom models mimicking the in vivo conditions, along with widespread microfluidic approaches to in vitro modeling. These devices create an in vitro biomimetic environment where the multi-step transmigration process can be imaged and quantified under mechanical and biochemical controlled conditions, including fluid dynamic settings, channel design, materials and surface coatings. This paper reviews the technological solutions recently proposed to model, observe and quantify leukocyte adhesion behavior under shear flow, with a final survey of high-throughput solutions featuring multiple parallel assays as well as thorough and time-saving statistical interpretation of the experimental results. (c) 2012 Elsevier Ltd. All rights reserved.

  • Details
  • Metrics
Type
review article
DOI
10.1016/j.jbiomech.2012.10.024
Web of Science ID

WOS:000314794200009

Author(s)
Bianchi, Elena  
Molteni, Raffaella
Pardi, Ruggero
Dubini, Gabriele
Date Issued

2013

Publisher

Elsevier

Published in
Journal Of Biomechanics
Volume

46

Issue

2

Start page

276

End page

283

Subjects

Microfluidics

•

Leukocyte adhesion and transmigration

•

Shear-stress dependent cellular in vitro assay

•

Parallel-plate flow chambers

•

High-throughput

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
CLSE  
Available on Infoscience
March 28, 2013
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/90875
Logo EPFL, École polytechnique fédérale de Lausanne
  • Contact
  • infoscience@epfl.ch

  • Follow us on Facebook
  • Follow us on Instagram
  • Follow us on LinkedIn
  • Follow us on X
  • Follow us on Youtube
AccessibilityLegal noticePrivacy policyCookie settingsEnd User AgreementGet helpFeedback

Infoscience is a service managed and provided by the Library and IT Services of EPFL. © EPFL, tous droits réservés