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  4. Continuous Photobleaching to Study the Growth Modes of Focal Adhesions
 
research article

Continuous Photobleaching to Study the Growth Modes of Focal Adhesions

de Beer, Alex G. F.
•
Majer, Guenter
•
Roke, Sylvie  
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2010
Journal of Adhesion Science and Technology

We combine total internal reflection fluorescence microscopy with continuous photobleaching to study the growth mode of focal adhesions in rat embryonic fibroblasts (REF-52). We measured GFP-labelled β3 integrin, which exhibits a diffusion rate of 10 -14-10 -13 m 2/s when it is not bound to a focal adhesion. We show that exchange of β3 integrin between focal adhesions and the surrounding membrane occurs only at the edges of the focal adhesion. We, therefore, conclude that focal adhesions show an edge-on/edge-off growth, in contrast to the bulk-on/bulk-off growth mode that is assumed in many theoretical treatments. © 2010 VSP.

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Type
research article
DOI
10.1163/016942410x508046
Web of Science ID

WOS:000284152300015

Scopus ID

2-s2.0-77957556151

Author(s)
de Beer, Alex G. F.
Majer, Guenter
Roke, Sylvie  
Spatz, Joachim P.
Date Issued

2010

Published in
Journal of Adhesion Science and Technology
Volume

24

Issue

13-14

Start page

2323

End page

2334

Editorial or Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
LBP  
Available on Infoscience
February 8, 2013
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/88698
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