000183110 001__ 183110
000183110 005__ 20190316235543.0
000183110 0247_ $$2doi$$a10.1109/TBCAS.2012.2220137
000183110 02470 $$2ISI$$a000313906500009
000183110 037__ $$aARTICLE
000183110 245__ $$aNew Approaches for Carbon Nanotubes-Based Biosensors and Their Application to Cell Culture Monitoring
000183110 269__ $$a2012
000183110 260__ $$bIeee-Inst Electrical Electronics Engineers Inc$$c2012$$aPiscataway
000183110 300__ $$a7
000183110 336__ $$aJournal Articles
000183110 520__ $$aAmperometric biosensors are complex systems and they require a combination of technologies for their development. The aim of the present work is to propose a new approach in order to develop nanostructured biosensors for the real-time detection of multiple metabolites in cell culture flasks. The fabrication of five Au working electrodes onto silicon substrate is achieved with CMOS compatible microtechnology. Each working electrode presents an area of 0.25 mm<sup>2</sup>	, so structuration with carbon nanotubes and specific functionalization are carried out by using spotting technology, originally developed for microarrays and DNA printing. The electrodes are characterized by cyclic voltammetry and compared with commercially available screen-printed electrodes. Measurements are carried out under flow conditions, so a simple fluidic system is developed to guarantee a continuous flow next to the electrodes. The working electrodes are functionalized with different enzymes and calibrated for the real-time detection of glucose, lactate, and glutamate. Finally, some tests are performed on surnatant conditioned medium sampled from neuroblastoma cells (NG-108 cell line) to detect glucose and lactate concentration after 72 hours of cultivation. The developed biosensor for real-time and online detection of multiple metabolites shows very promising results towards circuits and systems for cell culture monitoring.
000183110 6531_ $$aamperometric sensors
000183110 6531_ $$abiomarkers
000183110 6531_ $$acarbon nanotubes
000183110 6531_ $$acells
000183110 6531_ $$aelectrochemical device
000183110 700__ $$0242418$$g176461$$aBoero, Cristina
000183110 700__ $$0242419$$g183208$$aOlivo, Jacopo
000183110 700__ $$g167918$$aDe Micheli, Giovanni$$0240269
000183110 700__ $$aCarrara, Sandro$$g182237$$0242413
000183110 773__ $$j6$$tIEEE Transactions on Biomedical Circuits and Systems$$k5$$q479-485
000183110 8564_ $$uhttps://infoscience.epfl.ch/record/183110/files/06355640.pdf$$zn/a$$s1226087$$yn/a
000183110 909C0 $$xU11140$$0252283$$pLSI1
000183110 909CO $$particle$$qGLOBAL_SET$$ooai:infoscience.tind.io:183110$$pSTI$$pIC
000183110 917Z8 $$x112915
000183110 917Z8 $$x112915
000183110 937__ $$aEPFL-ARTICLE-183110
000183110 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000183110 980__ $$aARTICLE