Detecting particles flowing through interdigitated 3D microelectrodes

Counting cells in a large microchannel remains challenging and is particularly critical for in vitro assays, such as cell adhesion assays. This paper addresses this issue, by presenting the development of interdigitated three-dimensional electrodes, which are fabricated around passivated pillarshaped silicon microstructures, to detect particles in a flow. The arrays of micropillars occupy the entire channel height and detect the passage of the particle through their gaps by monitoring changes in the electrical resistance. Impedance measurements were employed in order to characterize the electrical equivalent model of the system and to detect the passage of particles in real-time. Three different geometrical micropillar configurations were evaluated and numerical simulations that supported the experimental activity were used to characterize the sensitive volume in the channel. Moreover, the signal-to-noise-ratio related to the passage of a single particle through an array was plotted as a function of the dimension and num ber of micropillars.


Published in:
2012 Annual International Conference Of The Ieee Engineering In Medicine And Biology Society (Embc), 5002-5005
Presented at:
34th Annual International Conference of the IEEE EMBS, San Diego, California, USA, August 28 - September 1, 2012
Year:
2012
Publisher:
New York, Ieee
ISSN:
1557-170X
ISBN:
978-1-4577-1787-1
Laboratories:




 Record created 2013-01-14, last modified 2018-09-13

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