Fluorescence Behavioral Imaging (FBI) tracks identity in heterogeneous groups of Drosophila

Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship partner choice in genetically mixed groups of Drosophila.


Publié dans:
PLOS One, 7, 11, e48381
Année
2012
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REOBehavior,WingX
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 Notice créée le 2012-09-28, modifiée le 2019-04-16

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