In vivo detection of brain Krebs cycle intermediate by hyperpolarized magnetic resonance

The Krebs (or tricarboxylic acid (TCA)) cycle has a central role in the regulation of brain energy regulation and metabolism, yet brain TCA cycle intermediates have never been directly detected in vivo. This study reports the first direct in vivo observation of a TCA cycle intermediate in intact brain, namely, 2-oxoglutarate, a key biomolecule connecting metabolism to neuronal activity. Our observation reveals important information about in vivo biochemical processes hitherto considered undetectable. In particular, it provides direct evidence that transport across the inner mitochondria membrane is rate limiting in the brain. The hyperpolarized magnetic resonance protocol designed for this study opens the way to direct and real-time studies of TCA cycle kinetics.Journal of Cerebral Blood Flow & Metabolism advance online publication, 19 September 2012; doi:10.1038/jcbfm.2012.136.


Published in:
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 32, 2108-2113
Year:
2012
Publisher:
New York, Nature Publishing Group
ISSN:
1559-7016
Keywords:
Laboratories:




 Record created 2012-09-25, last modified 2018-01-28

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