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research article
Exploiting Ligand-Protein Conjugates to Monitor Ligand-Receptor Interactions
2012
We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in cell extracts and fixed cells as well as the establishment of high-throughput assays. The different assays allow the analysis of strong and weak interactions. Conversion of ligands into SNAP-tag substrates thus provides access to a powerful toolbox for the analysis of their interactions with proteins.
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Name
Haruki et al PLOS One 2012.pdf
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openaccess
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2.02 MB
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Adobe PDF
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