Repository logo

Infoscience

  • English
  • French
Log In
Logo EPFL, École polytechnique fédérale de Lausanne

Infoscience

  • English
  • French
Log In
  1. Home
  2. Academic and Research Output
  3. Journal articles
  4. Observation of ion coalescence in Orbitrap Fourier transform mass spectrometry
 
research article

Observation of ion coalescence in Orbitrap Fourier transform mass spectrometry

Gorshkov, Mikhail V.
•
Fornelli, Luca  
•
Tsybin, Yury O.  
2012
Rapid Communications in Mass Spectrometry

RATIONALE Similar to other mass spectrometric technologies based on ion trapping in a spatially restricted area, the performance of Orbitrap Fourier transform mass spectrometry (FTMS) is affected by the interaction between the trapped ion clouds. One of the effects associated with Coulombic interaction inside the ion trap is the ion cloud coupling, known in ion cyclotron resonance (ICR) FTMS as coalescence, or a phase-locking phenomenon. Nevertheless, the direct observation of ion coalescence has not been reported for Orbitrap FTMS yet. METHODS We have performed experiments on ion coalescence with a pair of isobaric peptides in the state-of-the-art hybrid linear ion trap high-field compact Orbitrap Elite FT mass spectrometer using both standard and advanced signal processing modes. RESULTS For the instrument configuration employed in this work we found that ion coalescence occurs when two singly charged peptides with the mass difference of 22?mDa and molecular weight of about 1060?Da have the total abundance of at least 7.5*104 charges. CONCLUSIONS We experimentally demonstrate the existence of the ion coalescence phenomenon in Orbitrap FTMS for peptides for a wide range of total trapped ion population. Using the applicable modeling of the phase-locking threshold we estimate the effect of ion coalescence on the performance of Orbitrap FTMS. Copyright (C) 2012 John Wiley & Sons, Ltd.

  • Details
  • Metrics
Type
research article
DOI
10.1002/rcm.6289
Web of Science ID

WOS:000305574600007

Author(s)
Gorshkov, Mikhail V.
Fornelli, Luca  
Tsybin, Yury O.  
Date Issued

2012

Publisher

Wiley-Blackwell

Published in
Rapid Communications in Mass Spectrometry
Volume

26

Issue

15

Start page

1711

End page

1717

Subjects

Resolution

•

Accuracy

•

Proteins

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LSMB  
Available on Infoscience
June 28, 2012
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/82434
Logo EPFL, École polytechnique fédérale de Lausanne
  • Contact
  • infoscience@epfl.ch

  • Follow us on Facebook
  • Follow us on Instagram
  • Follow us on LinkedIn
  • Follow us on X
  • Follow us on Youtube
AccessibilityLegal noticePrivacy policyCookie settingsEnd User AgreementGet helpFeedback

Infoscience is a service managed and provided by the Library and IT Services of EPFL. © EPFL, tous droits réservés