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  4. Size- and Coating-Dependent Uptake of Polymer-Coated Gold Nanoparticles in Primary Human Dermal Microvascular Endothelial Cells
 
research article

Size- and Coating-Dependent Uptake of Polymer-Coated Gold Nanoparticles in Primary Human Dermal Microvascular Endothelial Cells

Freese, Christian
•
Gibson, Matthew I.
•
Klok, Harm-Anton  
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2012
Biomacromolecules

A library-orientated approach is used to gain understanding of the interactions of well-defined nanoparticles with primary human endothelial cells, which are a key component of the vasculature. Fifteen sequentially modified gold nanoparticles (AuNPs) based on three different core sizes (18, 35, 65 nm) and five polymeric coatings were prepared. The synthetic methodology ensured homogeneity across each series of particles to allow sequential investigation of the chemical features on cellular interactions. The toxicity of these nanoparticles, their uptake behavior in primary human dermal microvascular endothelial cells (HDMECs), and quantification of uptake were all investigated. The results of our studies indicated that high concentrations of gold nanoparticles (250 mu g/mL) were nontoxic and that the number of internalized nanoparticles was related to nanoparticle size and surface chemistry. In summary, the positive-charged ethanediamine-coated AuNPs were internalized to a greater extent than the negative- or neutral-charged AuNPs. Moreover, differences in the amounts of internalized AuNPs could be shown for the three neutral-charged AuNPs, whereas the uptake of hydroxypropylamine-coated particles was preferred compared with glucosamine-coated or PEGylated AuNPs. Hydroxypropylamine-coated AuNPs were found to be the most efficient neutral-charged particles in overcoming the endothelial cell barrier and entering the cell.

  • Details
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Type
research article
DOI
10.1021/bm300248u
Web of Science ID

WOS:000303951600035

Author(s)
Freese, Christian
Gibson, Matthew I.
Klok, Harm-Anton  
Unger, Ronald E.
Kirkpatrick, C. James
Date Issued

2012

Published in
Biomacromolecules
Volume

13

Start page

1533

End page

1543

Subjects

Fragmentation Chain Transfer

•

Mammalian-Cells

•

Cellular Uptake

•

In-Vitro

•

Cytotoxicity

•

Expression

•

Toxicity

•

Lines

•

Fate

•

Rats

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LP  
Available on Infoscience
June 8, 2012
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/81469
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