Nanopore Detection of Single Molecule RNAP–DNA Transcription Complex

In the past decade, a number of single-molecule methods have been developed With the aim of investigating single protein and nucleic acid interactions. For the first time we use solid-state nanopore sensing to detect a single E. coli RNAP DNA transcription complex and single E. coli RNAP enzyme. On the basis of their specific conductance translocation signature, we can discriminate and identify between those two types of molecular translocations and translocations of bare DNA. This opens up a new perspectives for investigating transcription processes at the single-molecule level.


Published in:
Nano Letters, 12, 3, 1157-1164
Year:
2012
ISSN:
1530-6992
Keywords:
Laboratories:




 Record created 2012-04-02, last modified 2018-03-17

n/a:
Download fulltext
PDF

Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)