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research article
Switchable fluorophores for protein labeling in living cells
Numerous synthetic fluorophores have been developed that can switch their spectroscopic properties upon interaction with other molecules or by irradiation with light. In recent years, protein-labeling techniques have been introduced that permit the specific attachment of such molecules to proteins of interest in living cells. We review here how the attachment of switchable fluorophores to selected proteins of interest via self-labeling protein tags enables new applications in different areas of biology and discuss how these molecules could be further improved.
Type
research article
Web of Science ID
WOS:000300034200004
Authors
Publication date
2011
Published in
Volume
15
Issue
6
Start page
768
End page
74
Peer reviewed
NON-REVIEWED
EPFL units
Available on Infoscience
January 20, 2012
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