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  4. Discovery and Analysis of Novel Metabolic Pathways for the Biosynthesis of Industrial Chemicals: 3-Hydroxypropanoate
 
research article

Discovery and Analysis of Novel Metabolic Pathways for the Biosynthesis of Industrial Chemicals: 3-Hydroxypropanoate

Henry, Christopher S.
•
Broadbelt, Linda J.
•
Hatzimanikatis, Vassily  
2010
Biotechnology And Bioengineering

Sustainable microbial production of high-value organic compounds such as 3-hydroxypropanoate (3HP) is becoming an increasingly attractive alternative to organic syntheses that utilize petrochemical feedstocks. We applied the Biochemical Network Integrated Computational Explorer (BNICE) framework to the automated design and evaluation of novel biosynthetic routes for the production of 3HP from pyruvate. Among the pathways generated by the BNICE framework were all of the known pathways for the production of 3HP as well as numerous novel pathways. The pathways generated by BNICE were ranked based on four criteria: pathway length, thermodynamic feasibility, maximum achievable yield to 3HP from glucose, and maximum achievable activity at which 3HP can be produced. Four pathways emerged from this ranking as the most promising for the biosynthesis of 3HP, and three of these pathways, including the shortest pathways discovered, were novel. We also discovered novel routes for the biosynthesis of 28 commercially available compounds that are currently produced exclusively through organic synthesis. Examination of the optimal pathways for the biosynthesis of these 28 compounds in E. coli revealed pyruvate and succinate to be ideal intermediates for achieving high product yields from glucose. Biotechnol. Bioeng. (C) 2010;106: 462-473, 2010 Wiley Periodicals, Inc.

  • Details
  • Metrics
Type
research article
DOI
10.1002/bit.22673
Web of Science ID

WOS:000277817300013

Author(s)
Henry, Christopher S.
Broadbelt, Linda J.
Hatzimanikatis, Vassily  
Date Issued

2010

Publisher

Wiley-Blackwell

Published in
Biotechnology And Bioengineering
Volume

106

Start page

462

End page

473

Subjects

Bnice

•

automated pathway generation

•

flux balance analysis

•

thermodynamic feasibility

•

Tmfa

•

Escherichia coli

•

Thermodynamic Analysis

•

Renewable Resources

•

Escherichia-Coli

•

Acid Production

•

Networks

•

Biodegradation

•

Information

•

Databases

•

System

•

Energy

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LCSB  
Available on Infoscience
December 16, 2011
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/75519
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