Superresolution Imaging using Single-Molecule Localization
Superresolution imaging is a rapidly emerging new field of microscopy that dramatically improves the spatial resolution of light microscopy by over an order of magnitude (similar to 10-20-nm resolution), allowing biological processes to be described at the molecular scale. Here, we discuss a form of superresolution microscopy based on the controlled activation and sampling of sparse subsets of photoconvertible fluorescent molecules. In this single-molecule-based imaging approach, a wide variety of probes have proved valuable, ranging from genetically encodable photoactivatable fluorescent proteins to photoswitchable cyanine dyes. These have been used in diverse applications of superresolution imaging: from three-dimensional, multicolor molecule localization to tracking of nanometric structures and molecules in living cells. Single-molecule-based superresolution imaging thus offers exciting possibilities for obtaining molecular-scale information on biological events occurring at variable timescales.
Keywords: superresolution microscopy ; single molecule ; Palm ; Storm ; Fpalm ; diffraction limit ; photoactivation ; photoactivatable fluorescent protein ; fluorescence imaging ; Green Fluorescent Protein ; Structured-Illumination Microscopy ; Optical Reconstruction Microscopy ; Hand-Over-Hand ; Living Cells ; Diffraction-Limit ; Quantum Dots ; In-Vivo ; 3-Dimensional Superresolution ; Live Cells
Record created on 2011-12-16, modified on 2016-08-09