To monitor cellular processes in individual cells, it is important to measure the concentrations of intracellular metabolites and to retrieve them for analysis. The use of functionalized polyelectrolyte microcapsules as intracellular sensors for in vivo reporting is persented. Capsules loaded with streptavidin-rhodamine, which was introduced into fibroblasts by electroporation, autonomously escaped from an endocytic compartment and efficiently recruited biotin-fluorescein from the cytosol. This work demonstrates the utility of polyelectrolyte microcapsules for intracellular capture of metabolites and eventually for drug delivery on an organismic level.
Type
research article
Web of Science ID
WOS:000284016700011
Authors
Studer, Deborah
•
Palankar, Raghavendra
•
Bedard, Matthieu
•
Winterhalter, Mathias
•
Springer, Sebastian
Publication date
2010
Published in
Volume
6
Start page
2412
End page
2419
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
December 16, 2011
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