Improved viability of freeze-thawed embryonic stem cells after exposure to glutathione

Adding a potent antioxidant, glutathione (GSH), to a cryoprotective solution consisting of dimethyl sulfoxide and ethylene glycol and/or postthaw culture medium significantly improved the postthaw viability of mouse embryonic stem cells. This effect, which was caused by a decrease in reactive oxygen species, was only induced by exposure of embryonic stem cells during cryopreservation. (Fertil Steril (R) 2010;94:2409-12. (C)2010 by American Society for Reproductive Medicine.)


Published in:
Fertility And Sterility, 94, 2409-2412
Year:
2010
Keywords:
Laboratories:




 Record created 2011-12-16, last modified 2018-09-13


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