Recognition and classification of red blood cells using digital holographic microscopy and data clustering with discriminant analysis

We propose to apply statistical clustering algorithms on a three-dimensional profile of red blood cells (RBCs) obtained through digital holographic microscopy (DHM). We show that two classes of RBCs stored for 14 and 38 days can be effectively classified. Two-dimensional intensity images of these cells are virtually the same. DHM allows for measurement of the RBCs' biconcave profile, resulting in a discriminative dataset. Two statistical clustering algorithms are compared. A model-based clustering approach classifies the pixels of an RBC and recognizes the RBC as either new or old based. The K-means algorithm is applied to the four-dimensional feature vector extracted from the RBC profile. (C) 2011 Optical Society of America


Published in:
Journal Of The Optical Society Of America A-Optics Image Science And Vision, 28, 1204-1210
Year:
2011
Keywords:
Laboratories:




 Record created 2011-12-16, last modified 2018-03-17


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