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research article
ZFN-Site searches genomes for zinc finger nuclease target sites and off-target sites
2011
Background: Zinc Finger Nucleases (ZFNs) are man-made restriction enzymes useful for manipulating genomes by cleaving target DNA sequences. ZFNs allow therapeutic gene correction or creation of genetically modified model organisms. ZFN specificity is not absolute; therefore, it is essential to select ZFN target sites without similar genomic off-target sites. It is important to assay for off-target cleavage events at sites similar to the target sequence.
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Name
1471-2105-12-152.pdf
Type
Publisher's version
Access type
openaccess
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3.44 MB
Format
Adobe PDF
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6fd21053b5a2abe2110b0235755e3835