Bubble cell for magnetic bead trapping in capillary electrophoresis
A bubble cell capillary classically used to extend the optical path length for UV-vis detection is employed here to trap magnetic beads. With this system, a large amount of beads can be captured without inducing a strong pressure drop, as it is the case with magnetic beads trapped in a standard capillary, thereby having less effect on the experimental conditions. Using numerical simulations and microscopic visualizations, the capture of beads inside a bubble cell was investigated with two magnet configurations. Pressure-driven and electro-osmotic flow velocities were measured for different amounts of protein-A-coated beads or C18-functionalized beads (RPC-18). Solid-phase extraction of a model antibody on protein-A beads and preconcentration of fluorescein on RPC-18 beads were performed as proof of concept experiments.
Keywords: Magnetic particle ; Bubble cell ; Solid-phase extraction ; Numerical simulation ; Finite element method ; Solid-Phase Extraction ; Spe-Ce ; Chip ; Immunoassay ; Particles ; Protein ; Line ; Separation ; Matrices ; Polymer
Record created on 2011-12-16, modified on 2016-08-09