Microchannel-guided capture antibody patterning on beads for multiple protein detection arrays

We present the rapid fabrication of custom protein micro-arrays, in less than 35 minutes, on the surface of immobilized beads using microchannel-guided flows driven by a single syringe pump. The commercially available beads provide robust micro-array surfaces to graft capture antibodies. The different target antigens are subsequently specifically bound and analysed in parallel in different microfluidic channels on a single micro-array chip. The presence of target molecules is detected as a fluorescent signal by realizing a sandwich immunocomplex on the specific capture antibody spot of the micro-array. With this technique, multiple proteins can be easily detected in a single channel.


Published in:
Proceedings of the 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS)
Presented at:
The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS), Seattle, Washington, USA, October 2-6, 2011
Year:
2011
Keywords:
Laboratories:




 Record created 2011-07-22, last modified 2018-09-13


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