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  4. Cell death detection and ionic homeostasis monitoring with digital holographic microscopy
 
conference paper

Cell death detection and ionic homeostasis monitoring with digital holographic microscopy

Pavillon, Nicolas  
•
Kühn, Jonas
•
Jourdain, Pascal  
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2011
Novel Optical Instrumentation for Biomedical Applications V
European Conferences on Biomedical Optics

Digital holographic microscopy is an interferometric technique enabling the measurement of the quantitative phase shifts induced by cell bodies. We correlate the phase signal measured on neurons with calcium imaging measured by fluorescence on cells loaded with Fluo-4, to monitor responses to glutamate challenges, which provoke well-known calcium increases through activation of various membrane receptors. A very good correspondence can be identified between the two signals, showing the links between the phase signal, being a measure of the intracellular dilution, and the calcium concentration within cells. We then check cell viability by employing propidium iodide (PI), a fluorescent indicator relying on the cell membrane integrity loss to assess cell death. Strong intracellular calcium concentration is indeed known to induce excitotoxic effects, potentially inducing cell death. This enables showing that some cells cannot sustain the calcium saturation identified in our measurements, leading to subsequent cell death.

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Type
conference paper
DOI
10.1117/12.889246
Web of Science ID

WOS:000293625400003

Author(s)
Pavillon, Nicolas  
Kühn, Jonas
Jourdain, Pascal  
Depeursinge, Christian  
Magistretti, Pierre  
Marquet, Pierre
Date Issued

2011

Publisher

SPIE

Published in
Novel Optical Instrumentation for Biomedical Applications V
Series title/Series vol.

Proceedings of SPIE

Volume

8090

Start page

809004

Subjects

[MVD]

URL

URL

http://spiedigitallibrary.org/proceedings/resource/2/psisdg/8090/1/809004_1
Editorial or Peer reviewed

NON-REVIEWED

Written at

EPFL

EPFL units
LNDC  
LOA  
Event nameEvent placeEvent date
European Conferences on Biomedical Optics

Munich

May 22-26, 2011

Available on Infoscience
June 21, 2011
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/68909
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