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research article
Real-Time Measurements of Protein Dynamics Using Fluorescence Activation-Coupled Protein Labeling Method
We present a fluorescence activation-coupled protein labeling (FAPL) method, which employs small-molecular probes that exhibit almost no basal fluorescence but acquire strong fluorescence upon covalent binding to tag-proteins. This method enables real-time imaging of protein labeling without any washout process and is uniquely suitable for real-time imaging of protein dynamics on the cell surface. We applied this method to address the spatiotemporal dynamics of the EGF receptor during cell migration.
Type
research article
Web of Science ID
WOS:000290363400043
Authors
Komatsu, Toru
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•
Okuno, Hiroyuki
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Bito, Haruhiko
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Inoue, Takanari
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Nagano, Tetsuo
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Urano, Yasuteru
Publication date
2011
Published in
Volume
133
Issue
17
Start page
6745
End page
6751
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
June 1, 2011
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