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  4. Identification of luminescent molecules comprises fluorescence correlation spectroscopy
 
patent

Identification of luminescent molecules comprises fluorescence correlation spectroscopy

Rigler, Rudolf  
•
Lasser, Theo  
•
Besse, Pierre-André  
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2005

The invention relates to a method and device for identifying luminescent molecules according to the fluorescence correlation spectroscopy method. The inventive method comprising the following steps: a) preparing a sample (12) containing luminescent molecules; b) illuminating the sample (12) using an optical excitation device (2, 4, 6, 8) comprising at least one light source, at least one, in particular, diffractive optical element (7) for splitting light passing therethrough into multiple beams, and a focussing optics (8) for focussing multiple light beams passing therethrough into multiple confocal volume elements; c) capturing emission radiation from the multiple confocal volume elements by means of a locally resolving sensor matrix arrangement (20), whereby the sensor matrix arrangement is a sensor matrix, which is comprised of avalanche photodiodes AD, is produced using IC technology, particularly CMOS technology, and is integrated in a sensor chip (20) with Geiger-mode wiring, and; d) processing the signals, which are provided by the avalanche photodiode matrix, by means of a signal processing and evaluation device preferably integrated in the sensor chip.

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Type
patent
EPO Family ID

34195729

34111975

38320201

Author(s)
Rigler, Rudolf  
Lasser, Theo  
Besse, Pierre-André  
Rochas, Alexis
Serov, Alexandre  
Gösch, Michael
Popovic, Radivoje
Subjects

Fluorescence correlation spectroscopy

Note

Alternative title(s) : (en) Method and device for identifying luminescent molecules according to the fluorescence correlation spectroscopy method

EPFL units
LOB  
DOICountry codeKind codeDate issued

DE20321480

DE

U1

2007-07-26

US2006226374

US

A1

2006-10-12

DE10336080

DE

A1

2005-03-03

WO2005017598

WO

A1

2005-02-24

Available on Infoscience
April 15, 2011
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/66487
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