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  4. Comparison of ALA- and ALA hexyl-ester-induced PpIX depth distribution in human skin carcinoma
 
research article

Comparison of ALA- and ALA hexyl-ester-induced PpIX depth distribution in human skin carcinoma

Dögnitz, Nora
•
Salomon, Denis
•
Zellweger, Matthieu  
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2008
Journal of photochemistry and photobiology.B.Biology

Photodynamic therapy (PDT) based on the use of photoactivable porphyrins, such as protoporphyrin IX (PpIX), induced by the topical application of amino-levulinic acid (ALA) or its derivatives, ALA methyl-ester (m-ALA), is a treatment for superficial basal cell carcinoma (BCC), with complete response rates of over 80%. However, in the case of deep, nodular-ulcerative lesions, the complete response rates are lower, possibly related to a lower bioavailability of PpIX. Previous in vitro skin permeation studies demonstrated an increased penetration of amino-levulinic acid hexyl-ester (h-ALA) over ALA. In this study, we tested the validity of this approach in vivo on human BCCs. An emulsion containing 20% ALA (w/w) and preparations of h-ALA at different concentrations were applied topically to the normal skin of Caucasian volunteers to compare the PpIX fluorescence intensities with an optical fiber-based spectrofluorometer. In addition, the PpIX depth distribution and fluorescence intensity in 26 BCCs were investigated by fluorescence microscopy following topical application of 20% ALA and 1% h-ALA. We found that, for application times up to 24h, h-ALA is identical to ALA as a PpIX precursor with respect to PpIX fluorescence intensity, depth of penetration, and distribution in basal cell carcinoma, but has the added advantage that much smaller h-ALA concentrations can be used (up to a factor 13). We observed a non-homogenous distribution in BCCs with both precursors, independent of the histological type and depth of invasion in the dermis.

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Type
research article
DOI
10.1016/j.jphotobiol.2008.07.012
Web of Science ID

WOS:000261966700004

Author(s)
Dögnitz, Nora
Salomon, Denis
Zellweger, Matthieu  
Ballini, Jean-Pierre
Gabrecht, Tanja
Lange, Norbert
van den Bergh, Hubert  
Wagnières, Georges  
Date Issued

2008

Publisher

Elsevier

Published in
Journal of photochemistry and photobiology.B.Biology
Volume

93

Issue

3

Start page

140

End page

8

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
GR-VDB  
GPM  
Available on Infoscience
January 27, 2011
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/63470
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