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research article

Spectroscopy of mobility-selected biomolecular ions

Papadopoulos, Georgios  
•
Svendsen, Annette
•
Boyarkin, Oleg V.  
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2011
Faraday Discussions

We describe here experiments that combine differential ion mobility, which separates conformational isomers of biomolecular ions, with electronic spectroscopy in a cold, radio-frequency ion trap. Although the low temperature attainable in a cold ion trap greatly simplifies the electronic spectra of large molecules, conformational heterogeneity can still be a significant source of congestion, complicating spectroscopic analysis. We demonstrate here that using differential ion mobility to separate gas-phase peptide conformers before injecting them into a cold ion trap allows one to decompose a dense spectrum into contributions from different conformational families. In the inverse sense, cold ion spectroscopy can be used as a conformation-specific detector for ion mobility, allowing one to separate an unresolved peak into contributions from different conformational families. The doubly protonated peptide bradykinin serves as a good test case for the marriage of these two techiques as it exhibits a considerable degree of conformational heterogeneity that results in a highly congested electronic spectrum. Our results demonstrate the feasiblity and advantages of directly coupling ion mobility with spectroscopy and provide a diagnostic of conformational isomerization of this peptide after being produced in the gas phase by electrospray.

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Type
research article
DOI
10.1039/C0FD00004C
Web of Science ID

WOS:000292977100013

Author(s)
Papadopoulos, Georgios  
Svendsen, Annette
Boyarkin, Oleg V.  
Rizzo, Thomas R.  
Date Issued

2011

Published in
Faraday Discussions
Volume

150

Start page

243

Subjects

laser spectroscopy

•

mass spectrometry

•

ion mobility

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LCPM  
Available on Infoscience
January 20, 2011
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/63187
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