Analytical description of STED microscopy performance

Stimulated emission depletion (STED) resolves fluorescent features that are closer than the far-field optical diffraction limit by applying a spatially modulated light field keeping all but one of these features dark consecutively. For estimating the efficiency of transient fluorophore darkening, we developed analytical equations considering the spatio-temporal intensity profile of the STED beam. These equations provide a quick analysis and optimization of the resolution and contrast to be gained under various conditions, such as continuous wave or pulsed STED beams having different pulse durations. Particular emphasis is placed on fluorescence fluctuation methods such as correlation spectroscopy (FCS) using STED.


Published in:
Optics Express, 18, 25, 26417
Year:
2010
Publisher:
Optical Society of America
ISSN:
1094-4087
Keywords:
Laboratories:


Note: The status of this file is: Involved Laboratories Only


 Record created 2010-12-05, last modified 2018-03-17

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