Multiple-Timescale Dynamics of Side-Chain Carboxyl and Carbonyl Groups in Proteins by C-13 Nuclear Spin Relaxation

Side-chain carboxyl and carbonyl groups play a major role in protein interactions and enzyme catalysis. A series of C-13 relaxation experiments is introduced to study the dynamics of carboxyl and carbonyl groups in protein side chains on both fast (sub-ns) and slower (mu s-ms) time scales. This approach is illustrated on the protein calbindin D-9k. Fast dynamics features correlate with hydrogen- and ion-binding patterns. We also identify chemical dynamics on mu s time scales in solvent-exposed carboxyl groups, most probably due to exchange between the carboxylate and carboxylic acid forms.


Published in:
Journal Of The American Chemical Society, 130, 15805-+
Year:
2008
Publisher:
American Chemical Society
ISSN:
0002-7863
Keywords:
Laboratories:


Note: The status of this file is: EPFL only


 Record created 2010-11-30, last modified 2018-03-17

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P284-Paquin-Ferrage-Mulder-Akke-Sidechain-Dynamics-JACS-130-15805-2008 - Download fulltextPDF
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