AFM Force Spectroscopy and Steered Molecular Dynamics Simulation of Protein Contactin 4

We use a single molecule atomic force spectroscopy combined with the steered molecular dynamics simulation to determine a mechanical behavior of neural cell adhesion protein contactin during its unfolding. Force curves typical for modular proteins were observed, showing at most four unfolding peaks. The analysis of force spectra performed within worm-like chain model of polymer elasticity showed the presence of three unfolding lengths. Small plateaus, most likely resulting from forced transitions within domains were observed for the first time. Steered molecular dynamics simulations help to determine atomistic picture of domain unfolding.


Published in:
Acta Physica Polonica A, 116, S156-S159
Presented at:
3rd National Conference on Nanotechnology, Warsaw, POLAND, Jun 22-26, 2009
Year:
2009
Publisher:
Polish Academy of Sciences Warsaw
ISSN:
0587-4246
Keywords:
Laboratories:




 Record created 2010-11-30, last modified 2018-03-17

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