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research article

Community and cultivation analysis of arsenite oxidizing biofilms at Hot Creek

Salmassi, T. M.
•
Walker, J. J.
•
Newman, D. K.
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2006
Environmental Microbiology

At Hot Creek in California, geothermally derived arsenite is rapidly oxidized to arsenate. This process is mediated by microorganisms colonizing the surfaces of submerged aquatic macrophytes in the creek. Here we describe a multifaceted approach to characterizing this biofilm community and its activity. Molecular techniques were used to describe the community as a function of 16S-rRNA gene diversity. Cultivation-based strategies were used to enumerate and isolate three novel arsenite oxidizers, strains YED1-18, YED6-4 and YED6-21. All three strains are β-Proteobacteria, of the genus Hydrogenophaga. Because these strains were isolated from the highest (i.e. million-fold) dilutions of disrupted biofilm suspensions, they represent the most numerically significant arsenite oxidizers recovered from this community. One clone (Hot Creek Clone 44) obtained from an inventory of the 16S rDNA sequence diversity present in the biofilm was found to be 99.6% identical to the 16S rDNA sequence of the isolate YED6-21. On the basis of most probable number (MPN) analyses, arsenite-oxidizing bacteria were found to account for 6-56% of the cultivated members of the community. Using MPN values, we could estimate an upper bound on the value of Vmax for the community of 1 × 10-9 μmole arsenite min-1 cell-1. This estimate represents the first normalization of arsenite oxidation rates to MPN cell densities for a microbial community in a field incubation experiment. © 2005 Society for Applied Microbiology and Blackwell Publishing Ltd.

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Type
research article
DOI
10.1111/j.1462-2920.2005.00862.x
Scopus ID

2-s2.0-33644960710

Author(s)
Salmassi, T. M.
Walker, J. J.
Newman, D. K.
Leadbetter, J. R.
Pace, N. R.
Hering, J. G.  
Date Issued

2006

Published in
Environmental Microbiology
Volume

8

Issue

1

Start page

50

End page

59

Editorial or Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
UPHCE  
Available on Infoscience
November 25, 2010
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/58665
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