Label-free second harmonic phase imaging of biological specimen by digital holographic microscopy

We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of a label-free biological tissue and discuss its added value to SHG microscopy. (C) 2010 Optical Society of America


Published in:
Optics Letters, 35, 24, 4102-4104
Year:
2010
Publisher:
Optical Society of America
ISSN:
0146-9592
Keywords:
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 Record created 2010-11-11, last modified 2018-03-18

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