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  4. Tissue-specific requirements for the proprotein convertase furin/SPC1 during embryonic turning and heart looping
 
research article

Tissue-specific requirements for the proprotein convertase furin/SPC1 during embryonic turning and heart looping

Constam, D. B.  
•
Robertson, E. J.
2000
Development

Furin, the mammalian prototype of a family of serine proteases, is required for ventral closure and axial rotation, and formation of the yolk sac vasculature. Here we show additionally that left-sided expression of pitx2 and lefty-2 are also perturbed in Furin-deficient embryos. These tissue abnormalities are preceded by a marked delay in the expansion of the definitive endoderm during gastrulation. Using a chimera approach, we show that Furin activity is required in epiblast derivatives, including the primitive heart, gut and extraembryonic mesoderm, whereas it is nonessential in the visceral endoderm. Thus, chimeric embryos, derived by injecting wild-type embryonic stem (ES) cells into fur(-/-) blastocysts, develop normally until at least 9.5 d.p.c. In contrast, Furin-deficient chimeras developing in the context of wild-type visceral endoderm fail to undergo ventral closure, axial rotation and yolk sac vascularization. Fur(-/-) cells are recruited into all tissues examined, including the yolk sac vasculature and the midgut, even though these structures fail to form in fur mutants. The presence of wild-type cells in the gut strikingly correlates with the ability of chimeric embryos to undergo turning. Overall, we conclude that Furin activity is essential in both extraembryonic and precardiac mesoderm, and in definitive endoderm derivatives.

  • Details
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Type
research article
Author(s)
Constam, D. B.  
Robertson, E. J.
Date Issued

2000

Published in
Development
Volume

127

Issue

2

Start page

245

End page

54

Subjects

Nuclear Proteins

Editorial or Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
UPCDA  
Available on Infoscience
October 21, 2010
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/55783
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