A simple and rapid technique for the detection of rifampin resistance in Mycobacterium leprae

The rifampin resistance of Mycobacterium leprae is due to missense mutations in the rpoB gene encoding the beta-subunit of the essential enzyme RNA polymerase. A rapid and very simple method has been developed to detect rifampin resistance in small numbers of M. leprae present in biopsies. It involves polymerase chain reaction amplification of a defined region of the rpoB gene followed by single-strand conformational polymorphism analysis (PCR-SSCP). The reliability of the method has been tested on a sample of known drug-resistant and susceptible isolates of M. leprae.


Published in:
International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association, 61, 4, 600-604
Year:
1993
ISSN:
0148-916X
Other identifiers:
Laboratories:




 Record created 2010-09-07, last modified 2018-12-03


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