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research article
A simple and rapid technique for the detection of rifampin resistance in Mycobacterium leprae
The rifampin resistance of Mycobacterium leprae is due to missense mutations in the rpoB gene encoding the beta-subunit of the essential enzyme RNA polymerase. A rapid and very simple method has been developed to detect rifampin resistance in small numbers of M. leprae present in biopsies. It involves polymerase chain reaction amplification of a defined region of the rpoB gene followed by single-strand conformational polymorphism analysis (PCR-SSCP). The reliability of the method has been tested on a sample of known drug-resistant and susceptible isolates of M. leprae.
Type
research article
PubMed ID
8151192
Authors
Publication date
1993
Volume
61
Issue
4
Start page
600
End page
604
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
September 7, 2010
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