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research article
Local comparison of the genomes of Mycobacterium tuberculosis and Mycobacterium leprae using the polymerase chain reaction
To facilitate comparison of the genome maps of Mycobacterium tuberculosis and M. leprae, sequence data from the M. leprae sequencing project were used to design primers suitable for the amplification of short segments from conserved genes by the polymerase chain reaction. In most cases, both organisms yielded products of identical size that were then used as probes for a comparative genomic walk. The hybridization data often, but not always, revealed a similar organization of various regions of both genomes. This approach should be useful for systematic comparisons of (myco)bacterial genomes.
Type
research article
PubMed ID
7590183
Authors
Publication date
1995
Published in
Volume
132
Issue
3
Start page
263
End page
9
Subjects
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
September 7, 2010
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