Local comparison of the genomes of Mycobacterium tuberculosis and Mycobacterium leprae using the polymerase chain reaction

To facilitate comparison of the genome maps of Mycobacterium tuberculosis and M. leprae, sequence data from the M. leprae sequencing project were used to design primers suitable for the amplification of short segments from conserved genes by the polymerase chain reaction. In most cases, both organisms yielded products of identical size that were then used as probes for a comparative genomic walk. The hybridization data often, but not always, revealed a similar organization of various regions of both genomes. This approach should be useful for systematic comparisons of (myco)bacterial genomes.


Published in:
FEMS microbiology letters, 132, 3, 263-9
Year:
1995
ISSN:
0378-1097
Keywords:
Other identifiers:
Laboratories:




 Record created 2010-09-07, last modified 2018-03-17


Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)