000151275 001__ 151275
000151275 005__ 20181203021958.0
000151275 022__ $$a0014-5793
000151275 02470 $$2PMID$$a10431837
000151275 0247_ $$2doi$$a10.1016/S0014-5793(99)00844-3
000151275 037__ $$aARTICLE
000151275 245__ $$aEvidence for a small anion-selective channel in the cell wall of Mycobacterium bovis BCG besides a wide cation-selective pore
000151275 269__ $$a1999
000151275 260__ $$c1999
000151275 336__ $$aJournal Articles
000151275 520__ $$aTwo channels were observed in extracts of whole Mycobacterium bovis BCG cells using organic solvents and detergents. The channels derived from organic solvent treatment had a single-channel conductance of about 4.0 nS in 1 M KCl in lipid bilayer membranes with properties similar to those of the channels discovered previously in Mycobacterium smegmatis and Mycobacterium chelonae. The channel was in its open configuration only at low transmembrane potentials. At higher voltages it switched to closed states that were almost impermeable for ions. Lipid bilayer experiments in the presence of detergent extracts of whole cells revealed another channel with a single-channel conductance of only 780 pS in 1 M KCl. Our results indicate that the mycolic acid layer of M. bovis BCG contains two channels, one is cation-selective and its permeability properties can be finely controlled by cell wall asymmetry or potentials. The other one is anion-selective, has a rather small single-channel conductance and is voltage-insensitive. The concentration of channel-forming proteins in the cell wall seems to be small, which is in agreement with the low cell wall permeability for hydrophilic solutes.
000151275 700__ $$aLichtinger, T
000151275 700__ $$aHeym, B
000151275 700__ $$aMaier, E
000151275 700__ $$aEichner, H
000151275 700__ $$0243892$$g177247$$aCole, S T
000151275 700__ $$aBenz, R
000151275 773__ $$j454$$tFEBS letters$$k3$$q349-55
000151275 909C0 $$xU11742$$0252302$$pUPCOL
000151275 909CO $$pSV$$particle$$ooai:infoscience.tind.io:151275
000151275 937__ $$aEPFL-ARTICLE-151275
000151275 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000151275 980__ $$aARTICLE